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[Original Javascript program by Eugen Buehler, |
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The common Tm formulas for calculating the theoretical Tm of an oligo are: The simlest formula: Tm = 4°C x (number of G’s and C’s in the primer) + 2°C x (number of A’s and T’s in the primer) Salt-Adjusted Tm Calculation Formula: Tm= 81.5 + 16.6 x (log10{[Na+] + [K+]}) + 0.41 x (%G+C) – 675/n Where: n is the number of nucleotides of the oligo [Na+] and [K+] are the final concentrations of the sodium and potassium ions in the reaction, respectively |
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