CRISPR knock-in protocol (1% DMSO trick)
Actin - HMM co-sedimentation assay.
Buffers and their pKa.
Chemical competent E. coli preparation.
Chromatin Immunoprecipitation (ChIP).
CIP-treatment.
Coomassie Blue Staining of Protein Gels.
E. coli lysis by lysozyme.
E. coli lysis by sonication.
F-actin prep (acetone powder).
FITC conjugation of Antibodies
Formation of Stable Colonies of Transformed Cells.
FREEZING ES CELLS.
Glycoprotein Staining.
Growing E coli for maxiprep.
GST column.
GST purif kit (Amersham).
GST Purification Protocol.
GSTfusions.
HMM prep.
IFM.
Immunofluorescence.
In gel kinase assay.
In vitro kinase assay with hot ATP.
IP for phospho-Western Blotting (endogenous protein).
IP guide1.
IP guide2.
Luciferase Assay Protocol with Promega's Kit.
MAXQUANT.
Microprep procedure for clone screening.
Myosin purification from worms.
Myosin Western.
NaOH and column cleaning.
Peptide kinase asay P81 protocol.
Phenol Chloroform DNA isolation.
Plasmid prep protocol.
Ponceau S - Reversible_staining_of_Western_Blots.
Potassium and Sodium buffers.
Preparation of SILAC media.
Protein Expression in E. coli.
Protein Extinction Coefficient Determination.
Protein Purification - Amersham Biosciences.
Protein purification from E. coli.
IP Protocols (Stressgen).
Pull-down.
Purification of Proteins from Sf9 cells.
Restriction Enzyme Digestion.
RNA isolation from cultured cells by using RNeasy kit (Qiagen).
SDS-PAGE
SILAC sample preparation
Silver Staining of Protein Gels.
Strippping Western Blots.
Synchronization protocols for human cells.
Transfection (using Lipofectamine 2000).
T-vector preparation for TA cloning.
Western Blotting (phospho-specific antibody).
Western Blot Strip Protocol.
Western Blotting (anti-His antibody).
YEAST TRANSFORMATION.
14-3-3 overlay assay.